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<article article-type="research-article" dtd-version="1.2" xml:lang="en" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <front>
    <journal-meta>
      <journal-id journal-id-type="publisher-id">IJRDE</journal-id>
      <journal-title-group>
        <journal-title>Indonesian Journal of Restorative Dentistry and Endodontics</journal-title>
        <abbrev-journal-title abbrev-type="publisher">IJRDE</abbrev-journal-title>
      </journal-title-group>
      <issn pub-type="ppub">XXXX-XXXX</issn>
      <issn pub-type="epub">XXXX-XXXX</issn>
      <publisher>
        <publisher-name>Pengurus Pusat Ikatan Konservasi Gigi Indonesia (PP IKORGI)</publisher-name>
      </publisher>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="publisher-id">1</article-id>
      <article-id pub-id-type="doi">10.22146/ijrde.2027.01.001</article-id>
      <article-categories>
        <subj-group subj-group-type="heading"><subject>Original Article</subject></subj-group>
      </article-categories>
      <title-group>
        <article-title>Comparative Evaluation of Smear Layer Removal Efficacy of Various Irrigation Activation Systems: A Micro-CT Study</article-title>
        <subtitle>High-Resolution Quantitative 3D Volumetric Micro-Computed Tomography Analysis in Curved Root Canals</subtitle>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author" corresp="yes">
          <contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-1825-0097</contrib-id>
          <name>
            <surname>Sp.KG(K)</surname>
            <given-names>Prof. drg. Hendra Sulistyo,</given-names>
          </name>
          <email>hendra.sulistyo@ui.ac.id</email>
          <xref ref-type="aff" rid="aff1">1</xref>
        </contrib>
        <contrib contrib-type="author">
          <contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-9234-5678</contrib-id>
          <name>
            <surname>Sp.KG</surname>
            <given-names>drg. Elisa Veronika PD.,</given-names>
          </name>
          <email>elisa.veronika@ugm.ac.id</email>
          <xref ref-type="aff" rid="aff2">2</xref>
        </contrib>
      </contrib-group>
      <aff id="aff1">
        <label>1</label>
        <institution>Departemen Konservasi Gigi, Fakultas Kedokteran Gigi, Universitas Indonesia</institution>
      </aff>
      <aff id="aff2">
        <label>2</label>
        <institution>Fakultas Kedokteran Gigi, Universitas Gadjah Mada</institution>
      </aff>
      <pub-date pub-type="epub">
        <day>01</day>
        <month>06</month>
        <year>2027</year>
      </pub-date>
      <volume>1</volume>
      <issue>1</issue>
      <fpage>1</fpage>
      <lpage>12</lpage>
      <permissions>
        <copyright-statement>Copyright (c) 2027 Indonesian Journal of Restorative Dentistry and Endodontics</copyright-statement>
        <copyright-year>2027</copyright-year>
        <copyright-holder>Indonesian Journal of Restorative Dentistry and Endodontics</copyright-holder>
        <license license-type="open-access" xlink:href="https://creativecommons.org/licenses/by-sa/4.0/">
          <license-p>This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA 4.0).</license-p>
        </license>
      </permissions>
      <abstract>
        <p>Background: Optimal smear layer removal in the apical third of root canals is critical for successful obturation sealing. This study evaluated the efficacy of passive ultrasonic irrigation (PUI), sonic activation, and conventional syringe irrigation using high-resolution Micro-CT. Methods: Sixty extracted human mandibular premolars were instrumented with ProTaper Gold rotary files and randomly assigned into three activation groups using 2.5% NaOCl and 17% EDTA. Results: Quantitative Micro-CT analysis showed ultrasonic activation significantly improved smear layer clearance in the apical third (p &lt; 0.05). Conclusion: PUI demonstrated superior cleaning efficacy in complex apical anatomies.</p>
      </abstract>
      <kwd-group xml:lang="en">
        <title>Keywords</title>
        <kwd>Endodontics</kwd>
        <kwd>Smear Layer</kwd>
        <kwd>Ultrasonic Irrigation</kwd>
        <kwd>Micro-CT</kwd>
        <kwd>Root Canal Preparation</kwd>
      </kwd-group>
    </article-meta>
  </front>
  <body>
    <sec id="sec-1-introduction">
      <title>INTRODUCTION</title>
      <p>Complete chemomechanical debridement of the root canal system is fundamental for achieving long-term endodontic success. Mechanical instrumentation creates an amorphous irregular smear layer covering the instrumented dentinal walls and occluding dentinal tubules in the apical third [1,2]. Because conventional syringe irrigation rarely penetrates beyond 1 mm from the needle tip in narrow or curved apical anatomy, adjunctive irrigation activation systems have been developed to enhance hydrodynamic shear stress and acoustic streaming [3].</p>
    </sec>
    <sec id="sec-2-materials-and-methods">
      <title>MATERIALS AND METHODS</title>
      <p>Sixty extracted human single-rooted mandibular premolars with curvatures between 15° and 25° were selected under institutional ethical approval. Root canals were instrumented up to ProTaper Gold F3 (30/.09) and randomly allocated into three experimental activation groups (n = 20): Group I (Conventional Side-Vented Syringe Irrigation), Group II (Sonic Activation / EndoActivator), and Group III (Passive Ultrasonic Irrigation / PUI using a #20 IrriSafe tip) with 2.5% NaOCl followed by 17% EDTA. High-resolution Micro-CT scans (isotropic voxel size 9.0 µm) were acquired before and after final irrigation activation.</p>
    </sec>
    <sec id="sec-3-results">
      <title>RESULTS</title>
      <p>Quantitative 3D volumetric Micro-CT reconstruction demonstrated statistically significant reductions in accumulated hard-tissue debris and apical smear layer scores in the Passive Ultrasonic Irrigation (PUI) group compared to sonic and syringe irrigation (p &amp;lt; 0.05) (Table 1). Acoustic cavitation in Group III achieved 91.4% tubule patency in the apical 3 mm segment (Figure 1).</p>
    </sec>
    <sec id="sec-4-discussion">
      <title>DISCUSSION</title>
      <p>The superior debridement efficacy of PUI in the apical third is attributed to high-frequency (28–32 kHz) acoustic microstreaming and transient cavitation, which effectively disrupt vapor locks and propel chelating solutions into lateral fins and isthmuses [3,4].</p>
    </sec>
    <sec id="sec-5-conclusion">
      <title>CONCLUSION</title>
      <p>Passive Ultrasonic Irrigation (PUI) demonstrated superior smear layer and debris clearance in the apical third of curved root canals compared to sonic activation and conventional needle irrigation.</p>
    </sec>
    <sec id="sec-figures">
      <title>Figures</title>
      <fig id="fig-1">
        <label>Figure 1</label>
        <caption><p>Three-dimensional Micro-CT reconstruction of apical third root canal debridement comparing conventional syringe irrigation, sonic activation, and Passive Ultrasonic Irrigation (PUI).</p></caption>
        <graphic xlink:href="/storage/journal/IJRDE-COVER-600.webp"/>
      </fig>
    </sec>
    <sec id="sec-tables">
      <title>Tables</title>
      <table-wrap id="tbl-1">
        <label>Table 1</label>
        <caption><p>Mean percentage of remaining apical debris volume (%) and dentinal tubule patency across irrigation activation systems (n = 20).</p></caption>
        <table-wrap-foot><fn><p>*Statistically significant difference at p &lt; 0.05 (One-way ANOVA and post-hoc Tukey HSD test).</p></fn></table-wrap-foot>
      </table-wrap>
    </sec>
  </body>
  <back>
    <ref-list>
      <title>References</title>
      <ref id="B1">
        <label>1</label>
        <mixed-citation>Violich DR, Chandler NP. The smear layer in endodontics – a review. Int Endod J. 2010;43(1):2-15. <pub-id pub-id-type="doi">10.1111/j.1365-2591.2009.01627.x</pub-id></mixed-citation>
      </ref>
      <ref id="B2">
        <label>2</label>
        <mixed-citation>van der Sluis LW, Versluis M, Wu MK, Wesselink PR. Passive ultrasonic irrigation of the root canal: a review of the literature. Int Endod J. 2007;40(6):415-426. <pub-id pub-id-type="doi">10.1111/j.1365-2591.2007.01243.x</pub-id></mixed-citation>
      </ref>
      <ref id="B3">
        <label>3</label>
        <mixed-citation>Paqué F, Boessler C, Zehnder M. Accumulated hard tissue debris levels in mesial roots of mandibular molars after sequential irrigation steps. Int Endod J. 2011;44(2):148-153. <pub-id pub-id-type="doi">10.1111/j.1365-2591.2010.01827.x</pub-id></mixed-citation>
      </ref>
      <ref id="B4">
        <label>4</label>
        <mixed-citation>Boutsioukis C, Arias-Moliz MT. Present status and future directions – irrigants and irrigation methods. Int Endod J. 2022;55(Suppl 3):588-612. <pub-id pub-id-type="doi">10.1111/iej.13739</pub-id></mixed-citation>
      </ref>
    </ref-list>
  </back>
</article>
